Hyderabad
08048036175
+919848399491
Laboratory Chemicals by MNC CHEMICALS SURGICALS

Cotton blue-lactophenol

Phone Number

08048036175

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Email Address mnc.naveen@gmail.com

Mon-Thu: 10 AM - 2 PM • Fri: 3 PM - 7AM

Address Correspondence Address: Shop No. 20, 2nd Floor, Srirama Tower, Bogulkunta, Telangana

Hyderabad, India, 500001

Description

Cotton blue-lactophenol, also known as Lactophenol Cotton Blue (LPCB), is a staining and mounting solution for microscopic identification of fungi, where cotton blue (aniline blue or methyl blue) stains fungal cell walls blue, while lactophenol preserves structures.​ Composition (Typical 100 mL Preparation) Cotton blue (aniline blue): 0.05–0.125 g.​ Phenol crystals: 20–25 g (disinfectant).​ Lactic acid: 20–50 mL (preservative).​ Glycerol: 40–100 mL (mountant).​ Distilled water: 20–50 mL.​ Preparation Protocol Dissolve cotton blue in distilled water; let stand overnight to settle insoluble dye.​ Dissolve phenol crystals in lactic acid (use gloves); add glycerol and stir.​ Filter the blue solution into the phenol mixture; mix and store at room temperature (matures 1–2 days).​ Properties and Uses Appearance: Clear blue solution.​ Staining: Fungal hyphae, spores, and structures stain deep blue against pale blue background.​ Applications: Wet mounts for fungal microscopy in mycology; kills, preserves, and stains in one step.​ Handling Light-stable but phenol-toxic; store sealed at RT. Use PPE; for lab use only.​ For catalog: “Cotton blue-lactophenol (LPCB), blue fungal stain/mountant, phenol-lactic acid-glycerol-aniline blue, for hyphae/spore microscopy; deep blue stain.”​

Model No. N-CH1643

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